Neisseria gonorrheae

Neisseria GONORRHEAE (Gonococcus)



Non-motile Gram-negative diplococci with fastidious growth requirements: capnophilics; Noncapsulated.



  • Gonorrhea
  • Pelvic inflammatory disease
  • Salpingitis in females
  • Ophthalmia neonatorum in infants



Human pathogens; no animal reservoir. This may be carried in genital tract, nasopharynx and anus. Spread by sexual or intimate contact.


Laboratory D1agnosis:

Sample Collection:

  • Urethral discharge collected by calcium alginate swab
  • Cervical swab from non-puerperal women

Transportation of sample:

Samples are transported in Amies transport medium.


Methods for identification of organism


 Direct methods:

  1. Gram staining:
    • Gram-negative diplococcic with concave adjacent sides (kidney shape), seen intracellularly  in polymorphoneuclear cells.
  1. Culture:
    • Culture media:
      • Chocolate agar,
      • Modified Thayer martin madium
    • Grow well at 37 c in moist atmosphere containing 5-10% co2
  1. Biochemical reactions:
    • Oxidase test positive
    • Sugar fermentation test,
      • Glucose   –   Acid no gas
      • Maltose  –      No reaction
  2. Antibiotic sensitivity test

Indirect methods:

  • Direct fluroscent antibody test and
  • Slide agglutination test