Diphtheria and Elek’s Gel Precipitation test




 

 

This presentation is about Diphtheria and Elek get test.

Elek gel precipitation test is Immunodiffusion in agar. It is used to detect toxin produced by Corynebacterium diphtheriae.

Corynebacterium diphtheriae are Gram positive bacilli.

They are Highly pleomorphic organisms with no particular arrangement.

They are having metachromatic granules at their polar regions.

They are non spore forming, and nonmotile bacterium. Corynebacterium diphtheriae is the pathogenic bacterium that causes vaccine preventable childhood disease, called diphtheria.

It is also known as the Klebs-Löffler bacillus, because it was discovered in 1884 by German bacteriologists Edwin Klebs and Friedrich Löffler.

Special stains like Albert’s stain and Ponder’s stain are used to demonstrate the metachromatic granules.

Here, as shown in image, culture isolate is plated in line and then filter paper shocked with anti toxin is placed perpendicular to inoculation line.

If culture isolate is having toxin, then it will make precipitation line as shown in image with black color.

If culture isolate does not have toxin production then there will be no any toxin antitoxin interaction, and no any line of precipitation, as shown in culture line plated on right side of plate.

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